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Synthesis of ( 2 H 5 ) indole‐3‐pyruvic acid
Author(s) -
BadenochJones Jane,
Summons Roger E.,
Letham David S.
Publication year - 1983
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580201203
Subject(s) - chemistry , indole test , indole 3 acetic acid , pyruvic acid , mass spectrometry , deuterium , chromatography , tryptophan , oxidase test , acetic acid , extraction (chemistry) , enzyme , amino acid , stereochemistry , organic chemistry , biochemistry , physics , quantum mechanics , auxin , gene
A method for the synthesis of deuterium‐labelled tryptophan and its enzymatic conversion to indole‐3‐pyruvic acid (( 2 H 5 )IPYA) using amino acid oxidase is described. ( 2 H 5 )IPYA can be used as an internal standard for the quantitation of IPYA by mass spectrometry in plant extracts and for estimating the breakdown of IPYA to indole‐3‐acetic acid (IAA) during sample extraction and purification. When used in conjunction with ( 2 H 2 ) IAA, ( 2 H 5 ) IPYA should allow more accurate quantitation of IAA in plant extracts.