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Tritiated peptides. Part 14 1 . Catalytic exchange labelling of various synthetic peptides
Author(s) -
Brundish Derek E.,
Combe M. Grace,
Wade Roy
Publication year - 1983
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580200712
Subject(s) - chemistry , tritium , labelling , residue (chemistry) , alanine , histidine , peptide , tritium illumination , specific activity , combinatorial chemistry , peptide synthesis , catalysis , stereochemistry , biochemistry , amino acid , enzyme , physics , nuclear physics
Several synthetic protected peptides have been catalytically exchangelabelled using tritium gas to a level between 0.1 and 12.5 Ci mmol −1 . High specific activities could be achieved for histidine‐containing peptides, and the factors influencing this have been investigated as have the location and stability of the introduced label. L‐Cysteinecontaining peptides gave rise to the corresponding peptides containing L‐alanine heavily labelled with tritium. The substitution pattern within the alanine residue has been established.