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The biosynthesis of three macrocyclic pyrrolizidine alkaloids retrorsine, seneciphylline and senecionine using senecio vulgaris
Author(s) -
Segall H. J.,
Brown C. H.,
Paige D. F.
Publication year - 1983
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580200602
Subject(s) - pyrrolizidine , chemistry , senecio , stereochemistry , botany , biology
Senecio vulgaris , a plant containing 3 macrocyclic pyrrolizidine alkaloids (PAs) was grown under atmospheric CO 2 , 14 CO 2 , and 13 CO 2 conditions. This report describes the plant growth chamber, the growing conditions the plants were subjected to and the amounts of macrocyclic PAs isolated from the plants. In addition, a number of radiolabelled experiments are described including a single 25 mCi pulse 14 CO 2 experiment, a dual 13 ,· 14 CO 2 experiment and an 800 mCi Pyrrolizidine Alkalolids 14 CO 2 experiment. The 14 C labelled macrocyclic PAs isolated from these experiments (retrorsine, seneciphylline and senecionine) are presently being used in various metabolism studies.