N‐succinimidyl propionate: Characterisation and optimum conditions for use as a tritium labelling reagent for proteins | Zendy

Tang Yui S | Zendy; Davis AnnMarie | Zendy; Kitcher J Philip | Zendy

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N‐succinimidyl propionate: Characterisation and optimum conditions for use as a tritium labelling reagent for proteins

Author(s) -

Tang Yui S,

Davis AnnMarie,

Kitcher J Philip

Publication year - 1983

Publication title -

journal of labelled compounds and radiopharmaceuticals

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.432

H-Index - 47

eISSN - 1099-1344

pISSN - 0362-4803

DOI - 10.1002/jlcr.2580200214

Subject(s) - chemistry , labelling , propionate , reagent , tritium , hydrolysis , lysine , radiochemistry , chromatography , nuclear chemistry , biochemistry , organic chemistry , amino acid , physics , nuclear physics

N‐Succinimidyl propionate [NSP] was synthesised and characterised as a pale yellow waxy solid. Rates of hydrolysis in buffers range pH 5–9 were determined. Optimum conditions for reaction with lysine were within the range pH 7 to 8. A protein, wheat germ agglutinin was labelled at pH 7.5 at room temperature for two hours. Rates of reaction of other substrates under these conditions were investigated.

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