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N‐succinimidyl propionate: Characterisation and optimum conditions for use as a tritium labelling reagent for proteins
Author(s) -
Tang Yui S,
Davis AnnMarie,
Kitcher J Philip
Publication year - 1983
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580200214
Subject(s) - chemistry , labelling , propionate , reagent , tritium , hydrolysis , lysine , radiochemistry , chromatography , nuclear chemistry , biochemistry , organic chemistry , amino acid , physics , nuclear physics
N‐Succinimidyl propionate [NSP] was synthesised and characterised as a pale yellow waxy solid. Rates of hydrolysis in buffers range pH 5–9 were determined. Optimum conditions for reaction with lysine were within the range pH 7 to 8. A protein, wheat germ agglutinin was labelled at pH 7.5 at room temperature for two hours. Rates of reaction of other substrates under these conditions were investigated.