Premium
Preparation of 14 C‐3‐hydroxyproline from 14 C‐proline by Peroxidation
Author(s) -
Ramaswamy Sengoda G.
Publication year - 1983
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580200210
Subject(s) - chemistry , hydroxyproline , proline , hydrogen peroxide , ascorbic acid , yield (engineering) , ferrous , amino acid , tritium , racemization , nuclear chemistry , organic chemistry , biochemistry , materials science , food science , metallurgy , physics , nuclear physics
Conditions are described for the treatment of proline with hydrogen peroxide, ascorbic acid, and ferrous ions to produce 3‐hydroxyproline in 5–6% yield. Several reaction products are formed, but they can be separated chromatographically to yield pure trans‐3‐hydroxyproline. Synthesis of the radioactive amino acid from 14 C‐L‐proline yields the pure isomer with specific activity identical to that of the starting proline. Identity of the desired product was established by chromatography, electrophoresis, and mass spectrum.