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Determination of inorganic radioiodine in solutions of 125 I‐labelled proteins
Author(s) -
Siuda Anjrzej,
Łucka Barbara
Publication year - 1981
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580180618
Subject(s) - chemistry , iodate , iodine , iodide , aqueous solution , solvent , ammonium , chromatography , iodine compounds , halogenation , ammonium iodide , inorganic chemistry , radiochemistry , organic chemistry
A rapid paper chromatography procedure using 33% w/v aqueous solution of ammonium sulphate as a solvent has been applied for routine determination of the proportion of 1251 present inorganic iodine in solutions containing 125I‐labelled proteins. R f values for protein, iodide and iodate correspond to 0.0, 0.5, and 0.9, respectively. The amount of inorganic iodine in the iodination mixture or in the iodinated protein preparation can be determined with good precision in less than 30 minutes.