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3‐[ 11 C]‐methyl‐D‐glucose, a potential agent for regional cerebral glucose utilization studies: Synthesis, chromatography and tissue distribution in mice
Author(s) -
Kloster G.,
MüllerPlatz C.,
Laufer P.
Publication year - 1981
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580180611
Subject(s) - chemistry , hydrolysis , high performance liquid chromatography , yield (engineering) , kidney , methylation , potassium , distribution (mathematics) , chromatography , specific activity , d glucose , radiochemistry , biochemistry , medicine , organic chemistry , enzyme , mathematical analysis , materials science , mathematics , metallurgy , gene
3‐[ 11 C]‐Methyl‐D‐glucose was synthetized by methylation via 11 CH 3 I of the potassium salt of diacetone‐D‐glucose; hydrolysis of the ketal groups with HCl yields 3‐[ 11 C]‐methyl‐D‐glucose. The radiochemical yield is 35% at a specific activity of 1.9 mCi/μmole. Synthesis time including purification by hplc is about 30 min. Up to 12.0 mCi have been obtained in injectable solution. The tissue distribution of 3‐[ 11 C]‐methyl‐D‐glucose was determined in mice at different times after an i.v. injection. All well‐perfused organs like lung, heart, liver, kidney and brain rapidly accumulate 3‐[ 11 C]‐methyl‐D‐glucose. The accumulation in brain is significant: at 15 min after application it reaches 6.2% dose/g organ.