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Direct isotope determination of drugs labeled with stable isotopes of hydrogen, carbon, and nitrogen by field desorption mass spectrometry
Author(s) -
Bahr U.,
Schulten H.R.
Publication year - 1981
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580180415
Subject(s) - chemistry , mass spectrometry , isotope , analytical chemistry (journal) , stable isotope ratio , field desorption , desorption , chromatography , nitrogen , mass spectrum , radiochemistry , ion , organic chemistry , adsorption , physics , quantum mechanics , ionization
By recording the molecular ion group with repetitive magnetic scanning and accumulation of the mass spectra in a multichannel analyser field desorption mass spectrometry was used to determine the isotopic abundances of three widely used drugs in their unlabeled and stable‐isotope labeled forms. The label content and statistical distribution of 2 H, 13 C and 15 N in analogs of an antitumor agent, cyclophosphamide and one of its metabolites 4‐ketocyclophosphamide and of two analgesics antipyrine and carbamazepine were investigated. Using nanogram amounts of sample the precision and accuracy of the measurements achieved were below ±1% on an absolute basis.