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A rapid and convenient method for specific 11 C‐labelling of synthetic polypeptides containing methionine
Author(s) -
Långström B.,
Sjöberg S.,
Ragnarsson U.
Publication year - 1981
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580180405
Subject(s) - labelling , chemistry , tripeptide , peptide , methionine , residue (chemistry) , yield (engineering) , methylation , peptide synthesis , liquid ammonia , protecting group , homocysteine , chromatography , amino acid , combinatorial chemistry , stereochemistry , ammonia , biochemistry , organic chemistry , materials science , alkyl , metallurgy , gene
11 C‐labelling of methionine residues in a synthetic peptide via the preparation of the corresponding protected, pure homocysteine peptide has been investigated. Complete deprotection of the peptide and specific methylation of the homocysteine residue can be performed in one step in liquid ammonia. As a first application of this method the synthesis of the tripeptide, Z‐Gly‐L‐Hcy (Bzl)‐Gly‐O‐Bzl, and its conversion to Gly‐Met‐Gly and the corresponding labelled Gly‐([ 11 C]‐methyl) ‐ Met‐Gly, is reported. Starting with the protected peptide the labelling was performed in 20 ± 5 min (starting with 11 CO 2 ), yielding the labelled peptide in 92 ± 5 % radiochemical yield. Analyses and preparative LC can be performed within 6 min.