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An enzymatic method for the preparation in high yield of [5‐ 14 C]‐ and [4,5,6‐ 14 C] glucose
Author(s) -
Longenecker J. P.,
Williams J. F.
Publication year - 1981
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580180305
Subject(s) - chemistry , transaldolase , glucose 6 phosphate isomerase , transketolase , triosephosphate isomerase , yield (engineering) , biochemistry , pentose phosphate pathway , dehydrogenase , enzyme , lactate dehydrogenase , pentose , dihydroxyacetone phosphate , fructose , isomerase , phosphogluconate dehydrogenase , glycerol , chromatography , glycolysis , glucose 6 phosphate dehydrogenase , materials science , fermentation , metallurgy
[5‐ 14 C]‐ and [4,5,6‐ 14 C] glucose were prepared from [2‐ 14 C]‐ and [U‐ 14 C] glycerol and fructose 6‐phosphate by reactions involving the enzymes glycerokinase, glycerol 3‐phosphate dehydrogenase, triose phosphate isomerase, transaldolase, lactate dehydrogenase and phosphoglucose isomerase. The authenticity of each of the 14 C‐labelled sugars was verified by chromatographic and enzymic procedures and the distribution of 14 C isotope confirmed by specific degradative procedures. These specifically labelled substrates are required for the quantitative estimation of the new pentose pathway reactions in liver, photosynthetic and some tumour tissues.