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Enzymatic synthesis and chromatographic purification of L‐3‐[ 11 C]‐lactic acid via D, L‐3‐[ 11 C]‐alanine
Author(s) -
Kloster G.,
Laufer P.
Publication year - 1980
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580170614
Subject(s) - chemistry , lactic acid , alanine , yield (engineering) , chromatography , transaminase , hydrolysis , enzyme , lactate dehydrogenase , high performance liquid chromatography , pyruvic acid , biochemistry , amino acid , bacteria , genetics , materials science , metallurgy , biology
D,L‐3‐[ 11 C]‐Alanine was prepared by methylation with 11 CH 3 I of tert butyl 2‐isocyanoacetate and subsequent hydrolysis. Enzymatic reaction using glutamate pyruvate transaminase and lactate dehydrogenase in a coupled reaction yields L‐3‐[ 11 C]‐lactic acid. The radiochemical yield is 45% for D,L‐alanine and 26.5% for L‐lactic acid at a specifie activity of 0.45 mCi/μmole. Synthesis time including purification by hplc is 23 min for D,L‐3‐[ 11 c]‐alanine and 45 min for L‐3‐[ 11 C]‐lactic acid, respectively.