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The preparation of 5′‐iodo‐ 125 I‐Δ 8 ‐THC; a radioligand for the radioimmunoassay of cannabinoids
Author(s) -
Pitt Colin G.,
Seltzman Herbert H.,
Setzer Susan R.,
Williams David L.
Publication year - 1980
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580170510
Subject(s) - chemistry , radioligand , sodium iodide , radioimmunoassay , iodide , hapten , radioligand assay , radiochemistry , sodium , sodium cyanide , chromatography , nuclear chemistry , organic chemistry , cyanide , biochemistry , antibody , binding site , immunology , biology
Nucleophilic exchange of 5′‐iodo‐Δ 8 ‐THC and sodium iodide‐125 afforded 5′‐iodo‐ 125 I‐Δ 8 THC with a maximum specific activity of 42 Ci/mmole. The specific activity could be increased to at least 100 Ci/mmole by iodide‐125 displacement of the corresponding 5′‐tosylate. A hapten 5′‐carboxy‐ 14 C‐Δ 9 ‐THC, for use in conjunction with this radioligand, was synthesized from 5′‐bromo‐Δ 8 ‐THC.