Premium
The enzymatic preparation of L(+)‐3‐hydroxy(3‐ 14 C)butyrate
Author(s) -
Dombrowski George,
Chao KuenLan,
Swiatek Kenneth R.
Publication year - 1980
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580170307
Subject(s) - chemistry , acetaldehyde , butyrate , alcohol dehydrogenase , stereospecificity , yield (engineering) , enzyme , dehydrogenase , ethanol , chromatography , organic chemistry , catalysis , fermentation , materials science , metallurgy
L(+)‐3‐hydroxy(3‐ 14 C)butyrate was prepared by treating the D, L‐racemic mixture with the stereospecific D‐(−)‐3‐hydroxybutyrate dehydrogenase enzyme. The unfavorable equilibrium position of the dehydrogenase was overcome by the addition of acetaldehyde and alcohol dehydrogenase. The D‐isomer was converted by this treatment to acetoacetate. The unreacted L(+)‐3‐hydroxy(3‐ 14 C)butyrate was then isolated by preparative silica gel chromatography in an overall yield of 65%.