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Non‐specific tritiation of some carcinogenic aromatic amines
Author(s) -
Breeman G. E. M.,
Kaspersen F. M.,
Westra J. G.
Publication year - 1978
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580140512
Subject(s) - chemistry , tritium , catalysis , trifluoroacetic acid , tritiated water , nitro , biphenyl , amino acid , tritium illumination , nitro compound , carcinogen , organic chemistry , radiochemistry , biochemistry , physics , alkyl , nuclear physics
2‐Aminofluorene, 4‐amino‐3‐methylbiphenyl, 4‐amino‐biphenyl and 4‐amino‐4′‐fluorobiphenyl were tritiated by acid catalyzed exchange of the corresponding nitro compounds followed by catalytic reduction. The exchange reactions were carried out by heating the nitro compounds in [ 3 H]‐trifluoroacetic acid with a catalytic amount of trifluoromethanesulphonic acid (TFMS). No loss of tritium could be detected during the conversion of the tritiated nitro compounds into the corresponding amines by catalytic hydrogenation. Incorporation into the ortho position is very low (< 4%). During the metabolic activation and binding of the tritiated N‐acetyl‐2‐aminofluorene to rat liver DNA in vivo, no tritium exchange occurred.