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Effect of reaction temperature on enzyme conversion of pyrimidine bases to nucleosides and 2′‐ deoxynucleosides
Author(s) -
Filip J.,
Přitasil L.,
Nejedlý Z.,
Veselý J.,
Čihák A.
Publication year - 1978
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580140220
Subject(s) - deoxyribonucleosides , chemistry , uracil , pyrimidine , thymine , uridine , nucleoside , yield (engineering) , deoxyribose , ribose , stereochemistry , nucleic acid , organic chemistry , enzyme , biochemistry , rna , dna , materials science , metallurgy , gene
Conversions of uracil to uridine and of uracil and thymine to the corresponding 2′‐deoxyribonucleosides catalyzed by the cell‐free extract of Escherichia coli B take place both at 37°C and 2°C. The yield of nucleosides formed during the short‐term incubation was higher at 37°C while during long‐term incubation it was higher at 2°C with the implication that the degradation of ribose‐ and 2′‐deoxyribose‐1‐phosphates in reaction mixtures kept at lower temperature is less considerable. The amount of 2′‐deoxyribonucleosides formed at 2°C was higher than that of ribonucleosides.