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An improved method for synthesis and purification of 125 I or 131 I labeled carrier‐free 5‐iodo‐2′‐deoxyuridine
Author(s) -
Keough W. G.,
Hofer Kurt G.
Publication year - 1978
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580140112
Subject(s) - chemistry , deoxyuridine , yield (engineering) , column chromatography , sephadex , halogenation , chromatography , high performance liquid chromatography , paper chromatography , radiochemistry , organic chemistry , biochemistry , dna , materials science , metallurgy , enzyme
Consistently high yields (up to 95%) of carrier‐free 5‐iodo‐2′‐deoxyuridine‐ 125 I (or 131 I) can be obtained by iodination of 2′‐deoxyuridine at varying concentrations of radioactive NaI (1–10 mCi), as long as the molarity of deoxyuridine in the reaction mixture is kept at or above 10 −2 M. At lower deoxyuridine molarities the reaction yield is significantly reduced. Several different purification procedures (Biogel P‐2 400 mesh or Sephadex G‐10 column chromatography, paper chromatography with water‐saturated ethyl acetate or water‐saturated butanol) are described. The two column chromatography systems yield iododeoxyuridine of > 95% chemical and radiochemical purity. The two paper chromatography systems are somewhat more involved, but yield 125 I or 131 I labeled 5‐iodo‐2′‐deoxyuridine of > 99% chemical and radiochemical purity.