Premium
Preparation of chloroacetaldehyde‐l‐ 14 C and its reaction with adenine and cytosine nucleotides
Author(s) -
Greenfield John C.,
Leonard Nelson J.,
Nystrom Robert F.
Publication year - 1976
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580120410
Subject(s) - chemistry , cytosine , nucleotide , adenine nucleotide , glycerol , stereochemistry , biochemistry , dna , gene
Chloroacetaldehyde, which reacts selectively with cytosine and adenine nucleotides to give fluorescent etheno‐bridged derivatives, was synthesized bearing a 14 C label. The chloroacetaldehyde l‐ 14 C, prepared from glycerol‐2‐ 14 C, was used to form etheno‐bridged adenine and cytosine nucleotides for enzyme studies and was applied to the chemical modification of tRNA.