Premium
Labelled polypeptides. v. synthesis of α‐melanotropin labelled with tritium on the tyrosine residue, via the dibromo intermediate
Author(s) -
Nikolics K.,
Teplán I.,
Medzihradszky K.
Publication year - 1976
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580120202
Subject(s) - chemistry , tritium , residue (chemistry) , tyrosine , peptide , stereochemistry , chemical synthesis , peptide synthesis , radiochemistry , tritiated water , combinatorial chemistry , organic chemistry , biochemistry , physics , nuclear physics , in vitro
A novel synthesis of α‐melanotropin labelled with tritium on the tyrosine residue ([Tyr‐3, 5‐ 3 H 2 ] ‐α‐melanotropin) is described. First, a precursor peptide, containing 3,5‐di‐bromotyrosine in position 2 ([Dbt 2 ]‐α‐melanotropin) is prepared, which can be converted into the labelled hormone by catalytic tritiation in the last step of the synthesis. By this procedure the tedious work with highly active labelled intermediates, and the storage of the tritiated compound. usually accompanied by significant autoradiolysis, can be avoided.