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Recombinant fragment of an antibody tailored for direct radioiodination
Author(s) -
Sedláček Juraj,
Fábry Milan,
Sieglová Irena,
Král Vlastimil,
Uhnáková Bronislava,
Múdra Marcela,
Kronrád Leo,
Sawicka Agnieszka,
Mikolajczak Renata,
Řezáčová Pavlína
Publication year - 2012
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.1945
Subject(s) - chemistry , recombinant dna , tyrosine , fragment (logic) , antibody , iodine , single chain variable fragment , solvent , biochemistry , stereochemistry , combinatorial chemistry , organic chemistry , immunology , gene , biology , computer science , programming language
This article presents the design and preparation of a single‐chain variable fragment (scFv) of an antibody comprising an auxiliary polypeptide segment that is rich in tyrosine. This protein shows a higher capacity to bind iodine radionuclide, as compared with the parental scFv. The tyrosine‐rich segment of the tailored protein is less hydrophobic (i.e. more solvent exposed) and can thus contribute to a redirection of the label from potentially sensitive scFv tyrosine residues.