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A gram‐scale synthesis of [3,4‐ 13 C 2, 1 α ,7‐ 2 H 2 ]cortisone
Author(s) -
Li Zizhong,
Heffner Peter,
Gong Yumin
Publication year - 2011
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.1938
Subject(s) - chemistry , regioselectivity , stereochemistry , double bond , deuterium , ring (chemistry) , medicinal chemistry , kinetic isotope effect , catalysis , organic chemistry , physics , quantum mechanics
A gram‐scale synthesis of [3,4‐ 13 C 2 ,1 α ,7‐ 2 H 2 ]cortisone from prednisone was developed. The deuterium atom at the C‐1 position was introduced through a regioselective and stereoselective deuteration of the 1,2‐double bond of the 1,4‐diene‐3‐one using Wilkinson's catalyst. After the oxidative cleavage of the A‐ring, two carbon‐13 atoms were introduced via acetylation of an A‐ring enol lactone with [1,2‐ 13 C 2 ]acetyl chloride. The steroidal A‐ring was then reconstructed to incorporate the carbon‐13 atoms into the C‐3 and C‐4 positions. The deuterium atom at C‐7 was introduced through a regioselective deuteration of the 6,7‐double bond of a 4,6‐diene‐3‐one intermediate using palladium on strontium carbonate. The M + 4 stable isotope labeled cortisone was thus prepared in ca . 4% overall yield. In addition, [3,4‐ 13 C 2 ,1 α ,7‐ 2 H 2 ]‐11‐dehydrocorticosterone, [3,4‐ 13 C 2 ,1 α ,7‐ 2 H 2 ]cortisol, and [3,4‐ 13 C 2 ,1 α ,7‐ 2 H 2 ]corticosterone were also prepared. Copyright © 2011 John Wiley & Sons, Ltd.