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Biosynthesis of glucose‐ d ‐ 13 C 6 from Hansenula polymorpha and methanol‐ 13 C
Author(s) -
Liu Zhanfeng,
Ren Zheng,
Xu Dagang,
Li Liangjun
Publication year - 2012
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.1935
Subject(s) - chemistry , methanol , yeast , trehalose , hydrolysis , fermentation , biosynthesis , carbon source , biochemistry , enzyme , stereochemistry , organic chemistry
A simple and effective method for synthesis of glucose‐ d ‐ 13 C 6 by fermentation using the methylotrophic yeast Hansenula polymorpha with 99% abundance methanol‐ 13 C is described. Using methanol‐ 13 C as a sole source of carbon, H. polymorpha can accumulate large amounts of α,α ‐trehalose‐ 13 C 12 under unfavourable growth conditions; the trehalose can then be hydrolysed to give glucose‐ d ‐ 13 C 6 with 98.5% abundance 13 C. Copyright © 2011 John Wiley & Sons, Ltd.
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