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Synthesis of carbon‐14, carbon‐13 and deuterium labeled forms of thioacetamide and thioacetamide S ‐oxide
Author(s) -
Sarma Diganta,
Hanzlik Robert P.
Publication year - 2011
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.1933
Subject(s) - thioacetamide , chemistry , metabolite , hepatotoxin , tautomer , oxide , deuterium , biochemistry , organic chemistry , quantum mechanics , physics , toxicity
Thioacetamide (TA) is a model hepatotoxin that undergoes metabolic activation via two successive S ‐oxidations. The ultimate toxic metabolite thioacetamide S,S ‐dioxide, or its tautomer acetimidoyl sulfinic acid CH 3 C(NH)SO 2 H, then acylates lysine side chains on cellular proteins leading to cellular dysfunction or death. To identify individual target proteins, quantitate the extent of their modification and elucidate the structural details of their modification, we required both radio‐labeled and stable‐labeled forms of TA and its intermediate metabolite thioacetamide S ‐oxide (TASO). The latter is stable when purified but can be difficult to isolate. Considering currently available isotopic precursors, we devised and report here methods for the synthesis and isolation of TA and TASO labeled with C‐14, C‐13, and/or deuterium. The methods are straightforward, utilize readily available precursors, and are amenable to small scale. Copyright © 2011 John Wiley & Sons, Ltd.