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Synthesis and purification of [2‐ 13 C]‐5‐fluorouracil
Author(s) -
Rangwala Huzaifa S.,
Giraldes John W.,
Gurvich Vadim J.
Publication year - 2011
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.1872
Subject(s) - chemistry , uracil , column chromatography , urea , silica gel , chromatography , fluorouracil , acute toxicity , high performance liquid chromatography , toxicity , combinatorial chemistry , organic chemistry , biochemistry , cancer , dna , medicine
[2‐ 13 C]‐5‐Fluoropyrimidine‐2,4( 1 H, 3 H)‐dione ([2‐ 13 C]‐5‐fluorouracil or [2‐ 13 C]‐5‐FU) is a potential diagnostic agent for measuring 5‐FU‐induced toxicity in cancer patients. It was prepared and purified with isotopic and chemical purity of>99% on a multigram scale in a two‐step synthesis from [ 13 C]‐urea. Preparative separation of [2‐ 13 C]‐FU and [2‐ 13 C]‐uracil was carried out by automated medium pressure silica gel column chromatography. The method is applicable to a broader range of 5‐FU isotopic analogs derived from labeled uracil. Copyright © 2011 John Wiley & Sons, Ltd.
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