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Labeling of PKC412 – a fermentative radiolabeling
Author(s) -
Burtscher P.,
Haecker H.,
Moenius T.
Publication year - 2010
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.1792
Subject(s) - chemistry , staurosporine , fermentation , biochemistry , enzyme , protein kinase c
Addition of [3‐ 14 C] D / L ‐tryptophan to the fermentation broth allowed radiofermentative labeling of staurosporine. The approach providing [4c,7a‐ 14 C 2 ]staurosporine in a range from 700 to 1300 MBq/mmol was straightforward and easy to handle as well as safe from a radioprotection point of view. The presented example underlines that close cooperation of microbiologists and radiochemists is a requisite for the success of this approach. Copyright © 2010 John Wiley & Sons, Ltd.