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Preparation of multideuterated 5‐deoxystrigol for use as an internal standard for quantitative LC/MS
Author(s) -
Ueno Kotomi,
Hanada Atsushi,
Yamaguchi Shinjiro,
Asami Tadao
Publication year - 2010
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.1789
Subject(s) - chemistry , deuterium , metabolite , chromatography , biochemistry , nuclear physics , physics
We prepared deuterium‐labeled 5‐deoxystrigol (5DS), a multifunctional plant second metabolite, using a simple set of procedures. The labeled compound contained mainly [3a,4,4,5,5,6′‐D 6 ]‐5DS and [D 5 ]‐5DS and did not contain unlabeled 5DS. Therefore, multideuterated 5DS can be used as an internal standard in liquid chromatography/mass spectrometry (LC/MS) assays to quantitate the amounts of 5DS in plants and root exudates. Copyright © 2010 John Wiley & Sons, Ltd.