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Simple single‐step single‐enzyme synthesis of [ 14 C]‐GSH
Author(s) -
de Keczer Steve A.,
Voronin Tatyana,
Yao Jennifer,
Zhang FangJie,
Masjedizadeh Mohammad R.
Publication year - 2010
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.1735
Subject(s) - chemistry , glutathione , cysteine , glycine , yield (engineering) , enzyme , glutathione synthetase , recombinant dna , thiol , glutathione disulfide , two step , biochemistry , stereochemistry , combinatorial chemistry , amino acid , materials science , metallurgy , gene
The tri‐peptide [ 14 C]‐glutathione ([ 14 C]‐GSH) was synthesized in a single step by GSH synthetase catalyzed reaction of L ‐γ‐glutamyl‐ L ‐cysteine and [ 14 C]‐glycine. Preparative reverse phase HPLC afforded [ 14 C]‐GSH in 30% yield and 98% purity. Preparation of GSH synthetase from E. coli via recombinant DNA and the interconversion of [ 14 C]‐GSH to the disulfide [ 14 C]‐GSSG for storage are discussed. Copyright © 2010 John Wiley & Sons, Ltd.
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