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Radiochemical and biological characteristics of 99m Tc‐difloxacin and 99m Tc‐pefloxacin for detecting sites of infection
Author(s) -
Motaleb M. A.
Publication year - 2010
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.1734
Subject(s) - pefloxacin , chemistry , ciprofloxacin , antibacterial agent , radiochemistry , nuclear chemistry , antibiotics , biochemistry , ofloxacin
The optimization of the radiolabeling yield of ciprofloxacin analogous, difloxacin and pefloxacin, with 99m Tc was described. At pH 4, difloxacin was labeled with 99m Tc with a labeling yield of 95.6% by adding 99m Tc to 5 mg difloxacin in the presence of 100 µg SnCl 2 ·2H 2 O whereas 99m Tc‐pefloxacin was labeled (98.1%) by adding 99m Tc to 4 mg pefloxacin in the presence of 50 µg SnCl 2 ·2H 2 O. The radiochemical purity for both labeled compounds was evaluated with ITLC and HPLC system. Biological distribution of 99m Tc‐difloxacin and 99m Tc‐pefloxacin was carried out in experimentally induced infection rats, in the left thigh, using Staphylococcus aureus . Both thighs of the rats were dissected and counted and the ratio of bacterial infected thigh/contralateral thigh was then evaluated. T/NT for both 99m Tc‐difloxacin and 99m Tc‐pefloxacin was found to be 5.5±0.5 and 4.9±0.3, respectively, which was higher than that of the commercially available 99m Tc‐ciprofloxacin. Copyright © 2010 John Wiley & Sons, Ltd.