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Application of 99 Mo/ 99m Tc alumina generator in the labeling of metoprolol for diagnostic purposes
Author(s) -
Amin A. M.,
ElAzony K. M.,
Ibrahim I. T.
Publication year - 2009
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.1661
Subject(s) - chemistry , metoprolol , pertechnetate , chloride , elution , yield (engineering) , high performance liquid chromatography , biodistribution , nuclear chemistry , technetium , radiochemistry , thin layer chromatography , chromatography , organic chemistry , medicine , materials science , metallurgy , cardiology , biochemistry , in vitro
Labeling of metoprolol by technetium‐99m in pertechnetate form ( 99m TcO − 4 ) eluted from a 99 Mo/ 99m Tc alumina generator in the presence of stannous chloride dihydrate was carried out via chelation reaction. The reaction parameters that affect the labeling yield such as metoprolol concentration, stannous chloride dihydrate concentration, reaction temperature, and pH of the reaction mixture were studied to optimize the labeling conditions. Using 1 GBq 99m TcO − 4 , 500 µg metoprolol as substrate dissolved in 500 µL phosphate buffer at pH 9 and 50 µL of stannous chloride as reducing agent (1 mg/mL) at 25°C for 30 min reaction time, a maximum radiochemical yield of 99m Tc‐metoprolol (92%) was obtained. 99m Tc‐metoprolol was characterized by thin layer chromatography (TLC) and by high pressure liquid chromatography (HPLC). The specific activity of 99m Tc‐metoprolol obtained was 888 MBq/1.88 mmol. The biological distribution in normal mice showed that 99m Tc‐metoprolol is rapidly concentrated after injection in the heart, which indicates its suitability for heart imaging. Copyright © 2009 John Wiley & Sons, Ltd.