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Novel and efficient detection of cAMP‐dependent protein kinase for the development of a protein biochip
Author(s) -
Park Sang Hyun,
Ko KyongCheol,
Choi Mi Hee
Publication year - 2009
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.1621
Subject(s) - biochip , chemistry , protein array analysis , protein chip , protein microarray , protein kinase a , substrate (aquarium) , kinase , proteomics , microbiology and biotechnology , phosphorylation , chromatography , dna microarray , biochemistry , nanotechnology , bioinformatics , biology , materials science , gene expression , gene , ecology
Protein microarrays have recently become a powerful research tool for proteomics and clinical investigations. A novel and efficient detection of cAMP‐dependent protein kinase for the development of a protein biochip has been established. The phosphorylation of a substrate by [γ‐ 33 P]ATP and [γ‐ 32 P]ATP was performed on a glass chip, and a facile detection of a kinase assay using 32 P and 33 P on a glass chip was accomplished by a radio‐TLC imaging scanner. Since the radio‐TLC detection method takes shorter detection time (2 min) than those required for X‐ray film or bioimage analyzer (6–12 h), the latter can be replaced. The detection by the radio‐TLC was proven to be rapid and suitable for a kinase assay using radioisotopes for developing a biochip. Copyright © 2009 John Wiley & Sons, Ltd.