Automated synthesis of  11 C‐β‐hydroxybutyrate by enzymatic conversion of  11 C‐acetoacetate using β‐hydroxybutyrate dehydrogenase | Zendy

Tremblay Sébastien | Zendy; Ouellet René | Zendy; Bénard François | Zendy; Cunnane Stephen C. | Zendy

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Automated synthesis of 11 C‐β‐hydroxybutyrate by enzymatic conversion of 11 C‐acetoacetate using β‐hydroxybutyrate dehydrogenase

Author(s) -

Tremblay Sébastien,

Ouellet René,

Bénard François,

Cunnane Stephen C.

Publication year - 2008

Publication title -

journal of labelled compounds and radiopharmaceuticals

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.432

H-Index - 47

eISSN - 1099-1344

pISSN - 0362-4803

DOI - 10.1002/jlcr.1504

Subject(s) - chemistry , chromatography , yield (engineering) , dehydrogenase , tetrahydrofuran , enzyme , nicotinamide adenine dinucleotide , ethanol , nad+ kinase , biochemistry , materials science , solvent , metallurgy

1‐[ 11 C]‐β‐hydroxybutyrate was produced by conversion from 1‐[ 11 C]‐acetoacetate using (D)‐β‐hydroxybutyrate dehydrogenase in the presence of nicotinamide adenine dinucleotide with purification by ion exchange column chromatography. Radiochemical yield at the end of the synthesis was 10% for a total synthesis time of 36 min. High‐performance liquid chromatography analysis showed ≤4% impurities, principally unconverted acetoacetate. Residual tetrahydrofuran (34±11 ppm) and ethanol (77±30 ppm) were well under the tolerable limits for human studies. Copyright © 2008 John Wiley & Sons, Ltd.

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