Stereoselective synthesis of L‐[ 15 N] amino acids with glucose dehydrogenase and galactose mutarotase as NADH regenerating system | Zendy

Chiriac Maria | Zendy; Lupan Iulia | Zendy; Bucurenci N. | Zendy; Popescu O. | Zendy; Palibroda N. | Zendy

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Stereoselective synthesis of L‐[ 15 N] amino acids with glucose dehydrogenase and galactose mutarotase as NADH regenerating system

Author(s) -

Chiriac Maria,

Lupan Iulia,

Bucurenci N.,

Popescu O.,

Palibroda N.

Publication year - 2008

Publication title -

journal of labelled compounds and radiopharmaceuticals

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.432

H-Index - 47

eISSN - 1099-1344

pISSN - 0362-4803

DOI - 10.1002/jlcr.1496

Subject(s) - chemistry , norleucine , stereospecificity , norvaline , dehydrogenase , valine , stereochemistry , stereoselectivity , leucine , enzyme , biochemistry , amino acid , isoleucine , galactose , catalysis

We have developed an efficient stereospecific enzymatic synthesis of L‐[ 15 N]‐valine, L‐[ 15 N]‐leucine, L‐[ 15 N]‐norvaline, L‐[ 15 N]‐norleucine and L‐[ 15 N]‐isoleucine from the corresponding α‐keto acids by coupling the reactions catalysed by leucine dehydrogenase and glucose dehydrogenase/galactose mutarotase. Giving high yields of L‐amino acids, the procedure is economical and easy to perform and to monitor at a synthetically useful scale (1–10 g). Copyright © 2008 John Wiley & Sons, Ltd.

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