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Stereoselective synthesis of L‐[ 15 N] amino acids with glucose dehydrogenase and galactose mutarotase as NADH regenerating system
Author(s) -
Chiriac Maria,
Lupan Iulia,
Bucurenci N.,
Popescu O.,
Palibroda N.
Publication year - 2008
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.1496
Subject(s) - chemistry , norleucine , stereospecificity , norvaline , dehydrogenase , valine , stereochemistry , stereoselectivity , leucine , enzyme , biochemistry , amino acid , isoleucine , galactose , catalysis
We have developed an efficient stereospecific enzymatic synthesis of L‐[ 15 N]‐valine, L‐[ 15 N]‐leucine, L‐[ 15 N]‐norvaline, L‐[ 15 N]‐norleucine and L‐[ 15 N]‐isoleucine from the corresponding α‐keto acids by coupling the reactions catalysed by leucine dehydrogenase and glucose dehydrogenase/galactose mutarotase. Giving high yields of L‐amino acids, the procedure is economical and easy to perform and to monitor at a synthetically useful scale (1–10 g). Copyright © 2008 John Wiley & Sons, Ltd.