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Synthesis of sulfur‐35 reagents for protein labeling
Author(s) -
Ren Sumei,
McNamara Paul,
Koharski David,
Hesk David,
Borges Scott
Publication year - 2007
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.1163
Subject(s) - reagent , chemistry , alkylation , lysine , yield (engineering) , aldehyde , sulfur , combinatorial chemistry , biochemistry , organic chemistry , amino acid , catalysis , materials science , metallurgy
Two 35 S reagents were developed to radiolabel proteins. The first reagent, a N ‐hydroxysuccinimide (NHS) ester (SMSB), acylates the ε‐amino group of lysine residues in proteins. The second reagent, an aldehyde (MSAPPA), labels lysine residues via reductive alkylation. Comparing the two methods, the reductive alkylation method labeled proteins over a broader pH range with higher overall radiochemical yield. The biological activity of the proteins did not change after labeling with these 35 S reagents. Copyright © 2007 John Wiley & Sons, Ltd.