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Bioavailability of 99m Tc‐Ha‐paclitaxel complex [ 99m Tc‐ONCOFID‐P] in mice using four different administration routes
Author(s) -
MeléndezAlafort Laura,
Riondato Mattia,
Nadali Anna,
Banzato Alessandra,
Camporese Davide,
Boccaccio Pasquale,
Uzunov Nikolay,
Rosato Antonio,
Mazzi Ulderico
Publication year - 2006
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.1107
Subject(s) - chemistry , paclitaxel , bioavailability , pharmacology , oral administration , biodistribution , spleen , chemotherapy , biochemistry , medicine , in vitro
Paclitaxel, an anti‐tumour drug, shows good results against breast and ovarian cancer. However, its therapeutic response is associated with toxic side‐effects caused by the agent used to dissolve it. Recently paclitaxel was linked to the linear polysaccharide hyaluronic acid (HA), showing good solubility, stabilization, localization and a reduction of cytotoxic side‐effects. To study potential therapeutic applications, HA‐paclitaxel bioconjugate (ONCOFID‐P) was labelled with 99m Tc by the addition of 99m Tc‐pertechnetate, SnCl 2 and sodium gluconate. The reaction mixture was incubated for 90 min at 65°C and purified by size exclusion chromatography. The obtained 99m Tc‐ONCOFID‐P had 100% radiochemical purity and was stable in a phosphate buffer dilution 1:100 for 6 h at 37°C. 99m Tc‐ONCOFID‐P bioavailability studies were carried out in healthy mice using four different administration pathways. The analysis showed that after intravenous administration more than 80% of the injected radiopharmaceutical was found in liver and spleen. Intraperitoneal, intravesical and oral administrations showed that all the 99m Tc‐ONCOFID‐P remained at the administration site. These results demonstrate that ONCOFID‐P administered intravenously could be used for liver metastasis therapy due to its high physiological and receptor‐specific liver uptake, while intravesical, intraperitoneal and oral administration of ONCOFID‐P could be used for local treatment of superficial cancers. Copyright © 2006 John Wiley & Sons, Ltd.