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Lectin isolated from Bothrops jararacussu venom induces IL‐10 release by TCD4 + cells and TNF‐α release by monocytes and natural killer cells
Author(s) -
Pires Weverson Luciano,
Kayano Anderson Makoto,
Castro Onassis Boeri,
Paloschi Mauro Valentino,
Lopes Jéssica Amaral,
Boeno Charles Nunes,
Pereira Soraya dos Santos,
Antunes Maísa Mota,
Rodrigues Moreno Magalhães S.,
Stábeli Rodrigo Guerino,
Fernandes Carla Freire Celêdonio,
Soares Andreimar Martins,
Zuliani Juliana Pavan
Publication year - 2019
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.ma1118-463r
Subject(s) - peripheral blood mononuclear cell , bothrops , biology , cytokine , tumor necrosis factor alpha , monocyte , immunology , superoxide , microbiology and biotechnology , in vitro , biochemistry , snake venom , venom , enzyme
BjcuL is a C‐type lectin isolated from Bothrops jararacussu snake venom with specificity for binding β‐ d ‐galactose units. BjcuL is not toxic to human peripheral blood mononuclear cells (PBMCs), but it inhibits PBMC proliferation and stimulates these cells to produce superoxide anions and hydrogen peroxide primarily via lymphocyte stimulation; it does not stimulate the production of nitric oxide and PGE 2 . The purpose of this study was to investigate the effect of BjcuL on PBMC activation with a focus on cytokine release modulating PBMC proliferation. The results showed for the first time that BjcuL coupled to FITC interacted with monocytes, B cells, natural killer (NK) cells, and with subpopulations of T cells. These cell‐cell interactions can lead to cell activation and inflammatory cytokines release, such as IL‐6 and TNF‐α, as well as the anti‐inflammatory cytokine IL‐10. In addition, TNF‐α release was attributed to NK cells and monocytes, whereas IL‐10 was attributed to TCD4 + and Treg cells when stimulated by BjcuL. The temporal cytokines profile produced by cells when stimulated with this lectin allows us to assert that BjcuL has immunomodulatory activity in this context.