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Granzyme B induced by Rv0140 antigen discriminates latently infected from active tuberculosis individuals
Author(s) -
Ouni Rym,
Gharsalli Houda,
Dirix Violette,
Braiek Amani,
Sendi Nadia,
Jarraya Afifa,
Douik El Gharbi Leila,
Barbouche MohamedRidha,
Benabdessalem Chaouki
Publication year - 2019
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.ma0318-117r
Subject(s) - granzyme b , immunology , granzyme , antigen , tuberculosis , biology , immune system , cytotoxic t cell , virology , cd8 , medicine , perforin , pathology , biochemistry , in vitro
Nearly two billion people are latently infected with Mtb (LTBI). Detection of LTBI with high risk to develop active tuberculosis (aTB) is considered the cornerstone to control the disease. The current challenge is to identify markers that better classify LTBI versus aTB. It has been previously shown that Rv0140, a reactivation‐associated antigen of Mtb , induces significantly higher IFN‐γ production in LTBI individuals as compared to aTB patients. Herein, we show that Rv0140 induces high granzyme B level by PBMCs derived from LTBI ( n = 34) as compared to aTB ( n = 18). Receiving operator characteristic (ROC) curves were used to evaluate the capacity of Rv0140 to discriminate between LTBI and aTB by measuring IFN‐γ and granzyme B secretion. Our results show that, in response to Rv0140, granzyme B seems to allow better discrimination of LTBI from aTB with areas under the curve (AUC) of 0.88 (95% CI 0.79–0.98) as compared to IFN‐γ with AUC of 0.85 (95% CI 0.74–0.96) even though CI overlap. Intracellular staining (ICS) experiments and the use of anti‐MHC I antibody showed that granzyme B is mainly produced by CD8+ T cells in response to Rv0140. Thus, we propose granzyme B as a host marker to help identify LTBI individuals.