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Role of mast cell leukotrienes in neutrophil recruitment and bacterial clearance in infectious peritonitis
Author(s) -
Malaviya Ravi,
Abraham Soman N.
Publication year - 2000
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.67.6.841
Subject(s) - mast cell , biology , leukotriene b4 , chemotaxis , immunology , microbiology and biotechnology , tumor necrosis factor alpha , in vivo , leukotriene , inflammation , receptor , asthma , biochemistry
Stimulated mast cells release a variety of chemotactic factors such as tumor necrosis factor α (TNF‐α) and leukotriene B 4 . Recent studies have shown that mast cell‐derived TNF‐α plays a critical role in host defense against Gram negative bacterial infections by the recruitment of neutrophils to the sites of infection. In the present study, we sought to investigate if mast cells release leukotriene (LT) B 4 in response to bacteria and, if so, to establish its in vivo relevance. We show that mast cells release significant amounts of LTB 4 and LTC 4 in response to exposure to FimH‐expressing type 1 fimbriated Escherichia coli in vitro. To test the functional significance of mast cell‐derived LTs during an E. coli infection in vivo , we examined the effect of a LT‐synthesis inhibitor, A‐63162, on bacterial clearance and neutrophil influx in an infectious peritonitis model in mast cell‐deficient mice (WBB6F 1 ‐W/W V ) and their normal congenic control (WBB6F 1 ‐+/+) mice. Our results show that a treatment with A‐63162 reduced neutrophil influx and bacterial clearance in the peritoneal cavities of mast cell‐sufficient but not ‐deficient mice. Thus, mast cell‐derived LTs contribute to host defense by mediating early neutrophil influx and bacterial clearance at sites of infection. J. Leukoc. Biol. 67:841–846; 2000.