Premium
Hypoxemia modifies circulating and exudate neutrophil number and functional responses in carrageenin‐induced pleurisy in the rat
Author(s) -
Tremblay PierreBenoît,
Macari David M.T.,
Martel Dominique,
Souich Patrick,
BarjaFidalgo Christina,
Marleau Sylvie
Publication year - 2000
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.67.6.785
Subject(s) - hypoxemia , exudate , nitric oxide , stimulation , granulocyte , neutrophile , basal (medicine) , medicine , pleurisy , immunology , endocrinology , inflammation , biology , pathology , pleural effusion , insulin
To assess the effect of hypoxemia on the responses of polymorphonuclear neutrophils (PMN) during an inflammatory response, rats were maintained in a low F i O 2 atmosphere (9% O 2 ) or room air for 12 h before intrathoracic injection of carrageenin or intradermal injections of agonists. After 4 h, hypoxemic rats had 50% more circulating PMN in blood and 25% less PMN in pleural exudate, whereas the number of PMN in skin biopsies did not differ from controls. Following hypoxemia, basal adhesion of blood PMN to serum‐coated plastic wells was unchanged, whereas fMLP‐stimulated adhesion was 50% greater. In contrast, basal adhesion of exudate PMN was 72% greater. In hypoxemic rats, exudate PMN produced 64% more PMA‐stimulated superoxide than blood PMN; furthermore, blood and exudate PMN produced 4.5‐ and 2‐fold more LPS‐stimulated nitric oxide than controls, respectively. These results show that a moderate level of hypoxemia may trigger mechanisms that will interfere with PMN emigration yet prime these cells for enhanced responses upon stimulation. J. Leukoc. Biol. 67: 785–792; 2000.