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A molecular analysis of NKT cells: identification of a class‐I restricted T cell‐associated molecule (CRTAM)
Author(s) -
Kennedy Jacqueline,
Vicari Alain P.,
Saylor Vicki,
Zurawski Sandra M.,
Copeland Neal G.,
Gilbert Debra J.,
Jenkins Nancy A.,
Zlotnik Albert
Publication year - 2000
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.67.5.725
Subject(s) - biology , natural killer t cell , cd8 , microbiology and biotechnology , natural killer cell , cd1 , granzyme b , mhc class i , cytotoxic t cell , t cell , immunoglobulin superfamily , granzyme a , mhc class ii , major histocompatibility complex , granzyme , chemokine , antibody , immunology , antigen , immune system , genetics , perforin , in vitro
cDNA library subtraction techniques were used to identify transcripts expressed by activated mouse αβPTCR + CD4 − CD8 − (double‐negative; DN) T cells, a subset of natural killer T (NKT) cells. The most frequent cDNAs identified included the chemokines TCA3, macrophage inflammatory protein‐1α (MIP‐1α), MIP‐1β, and lymphotactin (LPTN), the cytokines interleukin‐4 (IL‐4) and interferon‐γ (IFN‐γ), and a granzyme. We also identified a new member of the immunoglobulin superfamily (Ig‐SF). This molecule was designated class I‐restricted T cell‐associated molecule (CRTAM) as a result of its restricted expression pattern in T cells. Human CRTAM was also identified, and shares the same expression pattern as the mouse molecule. LPTN and CRTAM exhibit the same expression pattern in T cells, suggesting the existence of a gene expression program common to class I‐MHC‐restricted T cells. J. Leukoc. Biol. 67: 725–734; 2000.