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Analysis of interferon‐γ‐dependent and ‐independent pathways of macrophage activation
Author(s) -
Paulnock Donna M.,
Demick Karen P.,
Coller Susan P.
Publication year - 2000
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.67.5.677
Subject(s) - macrophage , biology , innate immune system , receptor , immune system , priming (agriculture) , interferon , microbiology and biotechnology , stimulation , scavenger receptor , immunology , in vitro , biochemistry , endocrinology , lipoprotein , botany , germination , cholesterol
Macrophages are a cellular cornerstone of the innate immune response. The outcome of macrophage activity during development of an immune response to microbes results from macrophage activation by both organism‐derived and host‐derived factors. In order to more fully understand the spectrum of responses expressed by macrophages when encountering these distinct stimuli, we investigated the similarities and differences between interferon‐γ receptor (IFN‐γR)dependent macrophage activation and stimulation of macrophages through the Type A1 scavenger receptor (SR). We observed distinct patterns of macrophage activation depending on the nature of the ligand. IFN‐γ and the SR ligand lipotechoic acid (LTA) induced largely non‐overlapping sets of genes. The use of two additional SR ligands, maleylated bovine serum albumin and the polydeoxynucleo‐tide poly dI:dC, revealed differences within SR activation‐induced gene expression. We also observed that priming with IFN‐γ resulted in an enhanced response to subsequent SR‐mediated activation. These results suggest that full potentiation of macrophage activity during development of an antimicrobial immune response is achieved by activation of these cells through multiple receptors. J. Leukoc. Biol. 67: 677–682; 2000.