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Neonatal neutrophil interaction with P‐selectin: contribution of P‐selectin glycoprotein ligand‐1 and sialic acid
Author(s) -
Tcharmtchi M. Hossein,
Smith C. Wayne,
Mariscalco M. Michele
Publication year - 2000
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.67.1.73
Subject(s) - p selectin , biology , neuraminidase , chinese hamster ovary cell , sialic acid , l selectin , venule , medicine , endocrinology , immunology , microbiology and biotechnology , receptor , biochemistry , platelet , cell adhesion molecule , enzyme , platelet activation , endothelium
Previously we had determined that neonatal neutrophils had decreased interaction with monolayers expressing P‐selectin compared to adult cells. In this study we examined the function of neonatal P‐selectin glycoprotein ligand‐1 (PSGL‐1). A rabbit polyclonal antibody directed against the amino terminus of human PSGL‐1 was produced and purified (3RB‐PSGL‐1). Neonatal neutrophils expressed the epitope recognized by 3RB‐PSGL‐1 and expression was decreased compared with adult neutrophils (20%, P < 0.05). In addition neonatal neutrophils had decreased interaction with Chinese hamster ovary (CHO)‐P‐selectin under both shear conditions and static adhesion ( P < 0.05). Treatment of both neonatal and adult neutrophils with 3RB‐PSGL‐1 similarly inhibited the interaction with P‐selectin monolayers under shear conditions, effects similar to treatment with O ‐sialoglycoprotein endopeptidase (OSGE). Neuraminidase treatment of neonatal and adult cells also markedly inhibited the interaction. In a detachment assay marked differences were noted between neonatal and adult cells treated with either 3RB‐PSGL‐1 or neuraminidase. Such treatments had little effect on adult neutrophils until shear stress exceeded 2.8 dynes/cm 2 . Treated neonatal neutrophils were exquisitely sensitive to shear stress with a marked decrease in interaction noted at a shear stress as low as 0.6 dynes/cm 2 . Thus the adhesive mechanisms that remain after treatment with neuraminidase or 3RB‐PSGL‐1 have a relatively low avidity and function less well in neonatal neutrophils compared to adult neutrophils. We speculate that this may account for the less efficient adhesion of neonatal neutrophils to P‐selectin under conditions of flow. J. Leukoc. Biol. 67: 73–80; 2000.