C/EBP regulates the promoter of the eosinophil‐derived neurotoxin/RNS2 gene in human eosinophilic cells | Zendy

Baltus Belinda | Zendy; Buitenhuis Miranda | Zendy; Dijk Thamar B. | Zendy; Vinson Charles | Zendy; Raaijmakers Jan A. M. | Zendy; Lammers JanWillem J. | Zendy; Koenderman Leo | Zendy; Groot Rolf P. | Zendy

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C/EBP regulates the promoter of the eosinophil‐derived neurotoxin/RNS2 gene in human eosinophilic cells

Author(s) -

Baltus Belinda,

Buitenhuis Miranda,

Dijk Thamar B.,

Vinson Charles,

Raaijmakers Jan A. M.,

Lammers JanWillem J.,

Koenderman Leo,

Groot Rolf P.

Publication year - 1999

Publication title -

journal of leukocyte biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.819

H-Index - 191

eISSN - 1938-3673

pISSN - 0741-5400

DOI - 10.1002/jlb.66.4.683

Subject(s) - biology , eosinophil , neurotoxin , gene , eosinophilic , microbiology and biotechnology , immunology , genetics , biochemistry , asthma

The eosinophil‐derived neurotoxin (EDN), a member of the mammalian ribonuclease family, is found in the large specific granules of human eosinophilic leukocytes. We have investigated the role of the C/EBP transcription factor family in the regulation of EDN promoter activity. Here we show that the C/EBP family is involved in intrinsic regulation of EDN promoter activity. We have identified a C/EBP binding site located at – 124 in the proximal promoter of the EDN gene. Mutation of this C/EBP site results in a decrease of promoter activity in HL‐60‐eos cells as well as in eosinophils differentiated in vitro from CD34 + cells. Different C/EBP proteins are able to bind to the C/EBP site as shown by gel shift assay. Our results indicate the importance of the C/EBP family in the regulation of the EDN gene in eosinophils. J. Leukoc. Biol. 66: 683–688; 1999.

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