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C/EBP regulates the promoter of the eosinophil‐derived neurotoxin/RNS2 gene in human eosinophilic cells
Author(s) -
Baltus Belinda,
Buitenhuis Miranda,
Dijk Thamar B.,
Vinson Charles,
Raaijmakers Jan A. M.,
Lammers JanWillem J.,
Koenderman Leo,
Groot Rolf P.
Publication year - 1999
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.66.4.683
Subject(s) - biology , eosinophil , neurotoxin , gene , eosinophilic , microbiology and biotechnology , immunology , genetics , biochemistry , asthma
Abstract The eosinophil‐derived neurotoxin (EDN), a member of the mammalian ribonuclease family, is found in the large specific granules of human eosinophilic leukocytes. We have investigated the role of the C/EBP transcription factor family in the regulation of EDN promoter activity. Here we show that the C/EBP family is involved in intrinsic regulation of EDN promoter activity. We have identified a C/EBP binding site located at – 124 in the proximal promoter of the EDN gene. Mutation of this C/EBP site results in a decrease of promoter activity in HL‐60‐eos cells as well as in eosinophils differentiated in vitro from CD34 + cells. Different C/EBP proteins are able to bind to the C/EBP site as shown by gel shift assay. Our results indicate the importance of the C/EBP family in the regulation of the EDN gene in eosinophils. J. Leukoc. Biol. 66: 683–688; 1999.