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The generation of human dendritic and NK cells from hemopoietic progenitors induced by interleukin‐15
Author(s) -
Bykovskaia Svetla.,
Buffo Mary,
Zhang Haifan,
Bunker Mark,
Levitt Mark L.,
Agha Mounzer,
Marks Stanley,
Evans Carol,
Ellis Peter,
Shurin Michael R.,
Shogan Jeffrey
Publication year - 1999
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.66.4.659
Subject(s) - biology , haematopoiesis , cd80 , interleukin 12 , cd34 , cd86 , interleukin 3 , microbiology and biotechnology , interleukin 15 , dendritic cell , cd40 , cytokine , progenitor cell , interleukin 21 , stem cell , immunology , interleukin , t cell , cytotoxic t cell , immune system , in vitro , biochemistry
Interleukin‐15 (IL‐15) is a pleiotropic cytokine that induces the generation and differentiation of lymphoid cells and shares many biological activities with IL‐2. We have shown here the development of dendritic cells (DC) from human CD34 + hemopoietic precursor cells cultured for 2–4 weeks with IL‐15 alone. DC generated with IL‐15 have typical morphological, immunocytochemical, phenotypic, and functional characteristics of mature DC. Dual flow cytometry analysis performed weekly demonstrated increasing co‐expression of CD1a or CD83 with HLA‐DR, CD80, CD86, IL‐2R α, β, and γ. Two populations of cells were distinguished among CD34 + progeny. Small and medium‐size cells were mainly natural killer (NK) cells (72.6–85.2% CD56 + ) and low numbers of DC (9.1–21.3% CD1a + ). Large cells were mostly DC (75.4–95.4% CD1a + ). Isolated CD34 + cells did not express IL‐2R subunits but after 2–3 days in culture with IL‐15, they were found to express IL‐2Rγ. Induced expression of IL‐2Rγ on CD34 + cells may explain the primary mechanism of IL‐15‐regulated differentiation of hemopoietic precursor cells. Thus, our data suggest that IL‐15 stimulates CD34 + cells to differentiate into NK and DC and may represent a new growth and survival factor for lymphoid DC. J. Leukoc. Biol. 66: 659–666; 1999.