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Differentiating agents regulate cathepsin B gene expression in HL‐60 cells
Author(s) -
Berquin Isabelle M.,
Yan Shiqing,
Katiyar Kamna,
Huang Li,
Sloane Bonnie F.,
Troen Bruce R.
Publication year - 1999
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.66.4.609
Subject(s) - biology , sodium butyrate , retinoic acid , microbiology and biotechnology , cathepsin b , gene expression , transfection , northern blot , regulation of gene expression , reporter gene , messenger rna , transcription (linguistics) , cellular differentiation , gene , biochemistry , enzyme , linguistics , philosophy
Abstract We utilized HL‐60 cells as a model system to examine the regulation of ctsb gene expression by differentiating agents. Inducers of monocytic differentiation [phorbol ester (PMA), calcitriol (D 3 ), and sodium butyrate (NaB)] and inducers of granulocytic differentiation [all‐ trans retinoic acid (RA) and 9‐ cis retinoic acid (9‐ cis RA)] increase ctsb mRNA levels in a dose‐dependent manner as determined by Northern blot hybridization. D 3 and retinoids exert additive effects, suggesting that these agents act in part through distinct pathways. Actinomycin D decay experiments indicate that D 3 , NaB, RA, and 9‐ cis RA do not alter mRNA stability. In contrast, PMA markedly increases the half‐life of ctsb mRNA. In transient transfection assays, PMA and NaB both stimulate transcription of the luciferase reporter gene placed under the control of ctsb promoter fragments. Thus, inducers of HL‐60 cell differentiation can regulate the expression of the ctsb gene at both transcriptional and posttranscriptional levels. J. Leukoc. Biol. 66: 609–616; 1999.