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SLC/Exodus2/6Ckine/TCA4 induces chemotaxis of hematopoietic progenitor cells: differential activity of ligands of CCR7, CXCR3, or CXCR4 in chemotaxis vs. suppression of progenitor proliferation
Author(s) -
Kim Chang H.,
Broxmeyer Hal E.
Publication year - 1999
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.66.3.455
Subject(s) - chemotaxis , biology , progenitor cell , cxcr4 , cxcr3 , haematopoiesis , microbiology and biotechnology , c c chemokine receptor type 7 , progenitor , cxcl14 , immunology , chemokine , chemokine receptor , stem cell , receptor , genetics , immune system
Chemokines induce chemotaxis of hematopoietic progenitor cells (HPC), and suppress their proliferation. In this study we report that SLC/Exodus2/6Ckine/TCA4 (hereafter termed SLC) is a chemoattractant for human CD34 + HPC. SLC mainly induces preferential chemotaxis of macrophage progenitors. We examined the chemotactic activity of CXCR3 ligands on CD34 + HPC because it has been reported that SLC is a potential ligand of CXC chemokine receptor, CXCR3, in addition to a CC chemokine receptor, CCR7. It was found that the CXCR3 ligands, MIG and interferon‐γ inducible protein‐10 (IP‐10), unlike SLC, did not induce chemotaxis of CD34 + HPC. In this regard, CCR7 ligands (SLC and CKβ‐11), but not IP‐10 and MIG, induce actin polymerization in CD34 + cells. On the other hand, CCR7 ligands and CXCR3 ligands, but not the CXCR4 ligand SDF‐1, showed inhibitory activity for proliferation of myeloid progenitor cells. Our results suggest that SLC is a potential trafficking factor for HPC, and that chemokines that bind CCR7, CXCR4, and CXCR3 have differential biological activities on HPC in terms of suppression and chemotaxis. J. Leukoc. Biol. 66: 455–461; 1999.