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Short exposure of intestinal epithelial cells to TNF‐α and histamine induces Mac‐1‐mediated neutrophil adhesion independent of protein synthesis
Author(s) -
Miyata Ryukou,
Iwabuchi Kazuhisa,
Watanabe Sumio,
Sato Nobuhiro,
Nagaoka Isao
Publication year - 1999
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.66.3.437
Subject(s) - histamine , cd18 , tumor necrosis factor alpha , biology , cell adhesion molecule , cycloheximide , cell adhesion , microbiology and biotechnology , cell–cell interaction , integrin alpha m , stimulation , adhesion , immunology , cell , chemistry , endocrinology , biochemistry , flow cytometry , protein biosynthesis , organic chemistry
Abstract Neutrophils play an important role in intestinal inflammation by interacting with intestinal epithelial cells. In this study, we evaluated neutrophil adhesion to intestinal epithelial cells using intestinal epithelial cell line HT29 stimulated with tumor necrosis factor α (TNF‐α) and histamine for a short time (30 min). The TNF‐α and histamine stimulation markedly increased neutrophil adhesion. The increased adhesion was inhibited by anti‐CD11b and anti‐CD18 monoclonal antibodies (mAbs), but not by anti‐CD11a and anti‐CD54 (ICAM‐1) mAbs. It is interesting that flow cytometric analysis revealed that ICAM‐1 expression on HT29 cells was not changed by TNF‐α and histamine stimulation. Moreover, the increased adhesion was inhibited by proteinase K treatment but not cycloheximide treatment of HT29 cells. Together these observations suggest that short exposure of HT29 cells to TNF‐α and histamine induces CD11b/CD18 (Mac‐1)‐dependent but CD11a/CD18 (LFA‐1)‐independent neutrophil adhesion to intestinal epithelial cells, and ICAM‐1 is not likely to be involved in the interactions. Furthermore, epithelial cell ligand(s) for neutrophils is likely protein molecule(s) that is expressed on the cell by stimulation independent protein synthesis. However, it is also possible that neutrophil activating factor(s), which stimulates neutrophils to bind with epithelial ligands via Mac‐1, is expressed by epithelial cells during stimulation. J. Leukoc. Biol. 66: 437–446; 1999.