Premium
Role of CD8α + and CD8α − dendritic cells in the induction of primary immune responses in vivo
Author(s) -
MaldonadoLópez Roberto,
De Smedt Thibaut,
Pajak Bernard,
Heirman Carlo,
Thielemans Kris,
Leo Oberdan,
Urbain Jacques,
Maliszewski Charlie R.,
Moser Muriel
Publication year - 1999
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.66.2.242
Subject(s) - biology , in vivo , immune system , cd8 , cytotoxic t cell , immunology , microbiology and biotechnology , primary (astronomy) , dendritic cell , in vitro , genetics , physics , astronomy
Data from adoptive transfer of mature dendritic cells (DC) indicate that they are responsible for the induction of primary immunity. Two subclasses of DC have been recently identified in spleen that differ in their phenotype and in certain regulatory features. In vitro , both subsets have the capacity to activate naive T cells, although CD8α + DC have been shown to induce T cell apoptosis and to stimulate lower levels of cytokines compared with CD8α − DC. The objective of this study was to analyze the function of these distinct DC types in vivo. Our results show that both subsets, pulsed extracorporeally with antigen and injected in the footpads of syngeneic mice, sensitize an antigen‐specific T cell primary response. However, CD8α + cells trigger the development of Th1‐type cells, whereas CD8α − DC induce a Th2‐type response. These observations suggest that the Th1/Th2 balance in vivo is regulated by the antigen‐presenting‐cells of the primary immune responses. J. Leukoc. Biol. 66: 242–246; 1999.