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Dendritic cells and MPIF‐1: chemotactic activity and inhibition of endogenous chemokine production by IFN‐γ and CD40 ligation
Author(s) -
Nardelli Bernardetta,
Morahan Diana K.,
Bong Gary W.,
Semenuk Mark A.,
Kreider Brent L.,
Garotta Gianni
Publication year - 1999
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.65.6.822
Subject(s) - chemokine , dendritic cell , biology , monocyte , microbiology and biotechnology , chemotaxis , immunology , cd40 , chemistry , inflammation , immune system , biochemistry , in vitro , cytotoxic t cell , receptor
We have examined the biological activity of the CC chemokine myeloid progenitor inhibitory factor 1 (MPIF‐1) on human dendritic cells. MPIF‐1 has chemotactic activity on dendritic cells derived from either peripheral blood monocytes or cord blood CD34 + progenitors. However, chemokine treatment did not induce further cell activation or maturation. In addition, MPIF‐1 is constitutively released by monocyte‐derived dendritic cells but not macrophages or monocytes (resting or stimulated). The proinflammatory stimuli lipopolysaccharide and tumor necrosis factor α, which induced the release of monocyte chemotactic protein‐1, macrophage inflammatory protein‐1α (MIP‐1α), MIP‐1β, and interleukin‐8, did not affect MPIF‐1 release. In contrast, CD40 ligation and interferon‐γ treatment, while stimulating the production of the other chemokines, caused a pronounced reduction of MPIF‐1 transcript and protein release. Thus, in dendritic cells the regulation of the production and release of MPIF‐1 is distinct in comparison to other CC and CXC chemokines. J. Leukoc. Biol. 65: 822–828; 1999.