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Vitamin E protects against polymorphonuclear leukocyte‐dependent adhesion to endothelial cells
Author(s) -
Yoshida Norimasa,
Yoshikawa Toshikazu,
Manabe Hiroki,
Terasawa Yoshimitsu,
Kondo Motoharu,
Noguchi Noriko,
Niki Etsuo
Publication year - 1999
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.65.6.757
Subject(s) - cd18 , integrin alpha m , protein kinase c , n formylmethionine leucyl phenylalanine , biology , umbilical vein , granulocyte , adhesion , stimulation , endocrinology , medicine , neutrophile , microbiology and biotechnology , inflammation , biochemistry , chemistry , immunology , receptor , phosphorylation , in vitro , organic chemistry
We evaluated the effects of α‐Toc on surface expression of CD11b/CD18 on polymorphonuclear leukocytes (PMN) stimulated with N ‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP) and oxidized low‐density lipoprotein (oxLDL). Incubation of PMN with fMLP (1 μM) or oxLDL (100 μg/mL) increased CD11b/CD18 expression; pretreatment with α‐Toc reduced in a dose‐dependent manner. PMN obtained from healthy adults ingesting 600 mg α‐Toc per day for 10 days were similarly incubated with fMLP or oxLDL; the surface level of CD11b/CD18 was inversely correlated with serum α‐Toc concentrations. Adherence of PMN to human umbilical vein endothelial cells was increased by fMLP or oxLDL stimulation but reduced by α‐Toc pretreatment or anti‐CD18 monoclonal antibodies. cAMP‐dependent protein kinase (PKA) and protein kinase C (PKC) activity in PMN was also assayed. A PKC inhibitor, but not a PKA inhibitor, suppressed CD11b/CD18 up‐regulation, and α‐Toc slightly decreased fMLP‐ and oxLDL‐induced activation of PKC. These results suggest that α‐Toc may prevent inflammation by both reducing CD11b/CD18 up‐regulation and decreasing PMN‐dependent adherence to EC. J. Leukoc. Biol. 65: 757–763; 1999.