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Selective antibody blockade of lymphocyte migration to mucosal sites and mast cell adhesion
Author(s) -
Palecanda Aiyappa,
Marshall Jean S.,
Li Xian,
Briskin Michael J.,
Issekutz Thomas B.
Publication year - 1999
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.65.5.649
Subject(s) - biology , immunology , mast cell , blockade , antibody , adhesion , lymphocyte , cell adhesion , microbiology and biotechnology , cell , receptor , biochemistry , chemistry , organic chemistry
The integrins α 4 β 7 and α 4 β 1 mediate adhesion to the mucosal addressin cell adhesion molecule‐1 (MAdCAM‐1) and the vascular cell adhesion molecule‐1 (VCAM‐1) and are important in T cell and allergic inflammatory reactions in the rat. The relative contributions of α 4 β 7 and α 4 β 1 in these reactions is unknown. To examine the role of α 4 β 7 in the rat a new mAb, TA‐6, was developed. TA‐6 inhibited adhesion to MAdCAM‐1 but not to VCAM‐1, a characteristic of α 4 β 7 adhesion, and immunofluorescence and immunoprecipitation studies were compatible with binding to α 4 β 7 . TA‐6 blocked rat lymphocyte adhesion to mesenteric lymph nodes and T cell migration to mucosal lymphoid tissues and it bound to rat mucosal mast cells. TA‐6 did not inhibit lymphocyte adhesion to peripheral lymph nodes and T cell migration to peripheral lymphoid tissues or cutaneous inflammatory sites, and was not expressed on connective tissue mast cells. J. Leukoc. Biol. 65: 649–657; 1999.