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Dendritic cell differentiation pathways of CD34 + cells from the peripheral blood of head and neck cancer patients
Author(s) -
Lathers Deanne M. R.,
Lubbers Eve,
Wright Mark A.,
Young M. Rita I.
Publication year - 1999
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.65.5.623
Subject(s) - biology , peripheral blood , cd34 , peripheral , immunology , cell , cancer , microbiology and biotechnology , cancer cell , cellular differentiation , cancer research , medicine , stem cell , genetics , gene
Patients with head and neck squamous cell carcinoma (HNSCC) have increased levels of immune‐suppressive peripheral blood CD34 + cells. This study showed that the peripheral blood CD34 + cells of HNSCC patients are capable of differentiating into dendritic cells. Because CD34 + cells can differentiate through several pathways into dendritic cell subpopulations, the intermediate cells through which the blood CD34 + cells of HNSCC patients differentiate were identified. After 6–7 days of culturing the CD34 + cells of HNSCC patients with granulocyte‐macrophage colony‐stimulating factor, stem cell factor, and tumor necrosis factor α, there appeared CD14 + CD1a + and a lesser proportion of CD14 − CD1a + cells resembling the precursor cells of the bipotential and committed dendritic cell differentiation pathways that have been described for cord blood CD34 + cells. To functionally analyze whether these populations were in fact precursor cells, they were isolated and cultured for an additional 10–12 days. Each of these populations was shown to function as precursor cells because they were able to develop into cells that resembled dendritic cells, al though a higher proportion developed from the CD14 − CD1a + cells. In contrast, expression of the dendritic activation/maturation marker CD83 was highest on the cells that developed from CD14 + CD1a + cells. Thus, the CD34 + cells whose levels are increased in HNSCC patients can develop into both committed and bipotential dendritic precursor cells, which can subsequently give rise to dendritic cells. J. Leukoc. Biol. 65: 623–628; 1999.